Evaluation of Nutritional, phytochemical, antioxidant and cytotoxic potential of Capsella bursa-pastoris, a wild vegetable from Potohar region of Pakistan


  • iqra riaz PMAS arid agriculture university Rawalpindi, Pakistan
  • yamin bibi PMAS arid agriculture university Rawalpindi, Pakistan
  • nabeela ahmed PMAS arid agriculture university Rawalpindi, Pakistan




Capsella bursa–pastoris, Potohar region of Pakistan, wild vegetable, phytochemical analysis


Capsella bursa-pastoris has been analyzed for nutritional composition, antioxidant and cytotoxic potential to reveal its nutraceutical and medicinal importance. Methanol and chloroform extracts were used for preliminary phytochemical analysis following quantification of total phenolic, flavonoid, tannins, saponins and alkaloids. Antioxidant activity was determined by using DPPH, phosphomolybdate and reducing power assays. Proximate and elemental analysis was performed by using standard methods. Brine shrimp larvae assay was used to determine cytotoxicity of chloroform and methanol extract. Chloroform and methanol extract of the plant indicated presence of 156µg/mg and 128µg/ml flavonoids respectively. Plant was found to have high carbohydrate and protein contents. High level mineral composition was determined containing Potassium, Sodium, Iron, Zinc and Copper. Results of antioxidant activity by DPPH method indicated IC50 of 235.37µg/ml and 552.01 µgml-1 for chloroform and methanol extract respectively. Brine shrimp larvae assay was used to determine the cytotoxicity resulting LD50 of 246.4469 μg/ml and 169.85μg/ml of methanol and chloroform extract respectively. Study inferred that Capsella bursa-pastoriscan can serve as a good source of protein and energy as well as micronutrients in the form of a leafy vegetable for human consumption.


Tsao, R.; Akhtar, M. H. Nutraceuticals and functional foods: Current trend in phytochemical antioxidant research. J. Food Agric. Environ. 2005, 3, 10-7.

. Hashmi, S. I.; Satwadhar, P. N.; Khotpal, R. R.; Deshpand, H. W.; Syed, K. A.; Vibhute, B. P. Rapeseed meal nutraceuticals. J. Cereals Oilseeds. 2010, 1, 43-54.

. Frison, E. A.; Smith, I. F.; Johns, T.; Cherfas, J.; Eyzaguirre, P. Agricultural biodiversity, nutrition, and health: making a difference to hunger and nutrition in the developing world. Food Nutr Bull. 2006, 27, 167–179.

Johns, T. E.; Eyzaguirre, P. B. Linking biodiversity, diet and health in policy and practice. Proc. Nutr. Soc. 2006, 65, 182–189.

Pardo-de-Santayana, M.; Tardio, J.; Blanco, E.; Carvalho, A. M.; Lastra, J. J.; Miguel, E. S.; et al. Traditional knowledge of wild edible plants used in the northwest of the Iberian Peninsula (Spain and Portugal): A comparative study. J. Ethnobiol. Ethnomed. 2007, 3, 16-27.

Podsedek, A. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT-Food Sci Technol. 2007, 40, 1-11.

Jayaprakasha, G. K.; Jaganmohan, R. Phenolic constituents from lichen ParmontremaS tuppeum. Hale and antioxidant activity. Z Naturforsch. 2000, C55, 1018-22

Racko, L.; Oblozinsky, M.; Kostalova, D.; Kettmann, V.; Bezakova, L. Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids. J. Inflamm. 2007, 4, 15–21.

Vinay, R. P.; Prakash, R. P.; Sushil, S. K. Antioxidant activity of some selected medicinal plants in western region of India. Adv. Biol. Res. 2010, 4, 23-26

Soleimanpour, S.; Sedighinia, F. S.; Afshar, A. S.; Zarif, R.; Asili, J.; Ghazvini, K. Synergistic Antibacterial Activity of Capsella bursa-pastorisand GlycyrrhizaglabraAgainst Oral Pathogens. ISO: Jundishapur. J. Microbiol. 2013, 6, 1-6.

Ajmal, S. M.; Mohammad, S.; Zahid, K.; Bakht, Z.; Habib, A.; Alam, M. Z. Ethnomedicinal and pyhtoeconomic elaboration Lilownai valley, District Shangla, Pakistan. Int. J. Pharm. 2012, 3, 164-169.

Jan, G.; Khan, M. A.; Gul, F.; Ahmad, M.; Jan, M.; Zafar, M. Ethnobotanical study of common weeds of dirkohistan valley, khyberpakhtoonkhwa, Pakistan. Pakistan J. Weed Sci. Res. 2010, 16, 81-88.

Grosso, C.; Vinholes, J.; Silva, L. R.; Guedes de Pinho, P.; Goncalves, R. F.; Valentao, P.; Jäger, A. K.; Andrade, P. B. Chemical composition and biological screening of Capsella bursa-pastoris. Rev. Bras. Farmacogn. 2011, 21, 635-644.

Duraipandiyan, V.; Ayyanar, M.; Ignacimuthu, S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu India. BMC Complement Altern. Med. 2006, 35, 1-7.

Huang, W.; Kun, Z. Cytotoxicity of a Plant Steroidal Saponin on Human Lung Cancer Cells. Asian Pac J Cancer Prev. 2011, 12, 513-517.

Mazid, M.; Khan, T. A.; Mohammad, F. Role of secondary metabolites in defense mechanisms of plants. ‎Exp. Biol. Med. 2011, 3, 232-249.

Ullah, M. F.; Khan, M. W.; Food as medicine: Potential therapeutic tendencies of plant derived polyphenolic compounds. Asian Pac J Cancer Prev. 2008, 9,187-195.

Vasantha, K.; Priyavardhini, S.; Soris, P. T.; Mohan, V. R. Phytochemical analysis and antibacterial activity of Kedrostis foetidissima (jacq.) Biosci. Discov. 2012, 3, 06-16.

Yuan, W. L.; Zhou, G.; Deng, P.; Wang, D.; Creech, SL (2011) Anthocyanins, Phenolics, and Antioxidant Capacity of Vaccinium L. in Texas, USA Pharmaceutical 2, 11-23.

Ali, N.; Syed, A. S.; Shah, I. W.; Mehreen, A. G.; Khan, I. Cytotoxic and anthelmintic potential of crude saponins isolated from Achillea Wilhelmsii C. Koch and Teucrium Stocksianum boiss. BMC Complement Altern. Med. 2011, 11, 106. doi: 10.1186/1472-6882-11-106

Hala, U.; Gali-Muhtasib, I. H.; Younes, J. J.; Karchesy, Marwan, E. E. Plant Tannins Inhibit the Induction of Aberrant Crypt Foci and Colonic Tumors by 1, 2-Dimethylhydrazine in Mice. Nutr. Cancer 2011, 39, 108-116.

Iyaka, Y, A. Concentration of Cu and Zinc in some fruits and vegetables commonly available in North-Central zone of Nigeria. Elect. J. of Environ. Agri. & Food Chemis. 2007, 6, 2150-2154.

Singh, U.; Kochhar, A.; Boora, R. Proximate composition, available carbohydrates, dietary fibers and anti-nutritional factors in BAEL (Aegle maemelos L.) leaf, pulp and seed powder. Int. J. Sci. Res. 2012, 2, 1-4.

Fasuyi, A. O. Nutritional potentials of some tropical vegetable meals. Chemical characterization and functional properties. Afr. J. Biotechnol. 2006, 5, 49-53.

Rodriguez, R. A.; Jimenez, J.; Fernandez-Bolanos, R.; Guillen, Heredia, A. Dietary fibers from vegetable products as source of functional ingredients. Trends Food Sci. Technol. 2006, 17, 3-15

Idris, S.; Ndamitso, M. M.; Yisa, J.; Dauda, B. E. N.; Jacob, J. O. The proximate and mineral composition of the leaves and stems of Balanitesaegyptiaca. Int. J. Advan. Biolog. Res. 2010, 2, 76-87.

Camera, F.; Amaro, C. A. Nutritional aspect of zinc availability. Int. J. Food Sci. Nutr. 2003, 47, 143 – 151.

McDonald, P.; Edward, R. A.; Greenhalti, F. D.; Morgan, C. A. (1995) Animal nutrition. Prentices Hall, London. Edition Prentice Hall, pp 101–122.

Thambiraj, J.; Paulsamy, S. In vitro antioxidant potential of methanol extract of the medicinal plant, Acacia caesia (L.) Wild. Asian Pac. J. Trop. Biomed. 2012, 2, S732–S736.

Vishnu, R.; Nisha, R.; Jamuna, S.; Paulsamy, S. Quantification of total phenolics and flavonoids and evaluation of in vitro antioxidant properties of methanolic leaf extract of Tarennaasiatica - an endemic medicinal plant species of Maruthamali hills, Western Ghats, Tami Nadu. J. Res. Plant Sci. 2013, 2,196–204.

Johnson, W. T. (2005) In Nutritional and Neuroscience, H.R. Lieberman, R.B. Kanarek, and C. Prasad (Ed). Taylor & Francis, Boca Raton, pp17.

Oliveira, I.; Sousa, A.; Ferreira, I.; Bento, A.; Estevinho, L.; Pereira, J. A. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglansregia L.) green husks. Food Chem. Toxicol. 2008, 46, 2326–2331.

Gulcin, I.; Oktay, M.; Kirecci, E.; Kufrevioglu, O. I. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chem. 2003, 83, 371−382.