Evaluation of partial characteristics of the strain Enterococcus faecalis P3 isolated from peacock feces in vitro

Yu Zhang, Wei Zheng, Jian Ni, Ting-Ting Xie, Ling Wang, Lu-E Shi, Zhen-Xing Tang

Abstract


In this study, the potential probiotic properties such as NaCl tolerance, acid tolerance, simulated gastro-intestinal juicetolerance, adhesion ability to Hep-2 cells, antibiotic susceptibility and antimicrobial activity against selected pathogens, of the strain E. faecalis P3 identified in our group were evaluated in vitro. The results showed that E. faecalis P3 had
tolerance to NaCl. The viability was kept higher than 8 log CFU/mL at 2-5 % concentration of NaCl during 24 h incubation. E. faecalis P3 grew well in acid condition (pH 1.8-6.2) for 24 h incubation. The viable numbers decreased with the increase of incubation time in simulated gastro-intestinal juices. The viable numbers were kept higher than 10 log CFU/mL in simulated gastric juice (SGJ) pH 2.5 after 2 h incubation. Furthermore, E. faecalis P3 was able to adhere
to Hep-2 cells. The results of antibiotic susceptibility indicated E. faecalis P3 was sensitive to most of the clinically important antibiotics. E. faecalis P3 had good inhibition ability on Staphylococcus aureus. In conclusion, E. faecalis P3 appeared to be a good candidate for use as a probiotic agent in food or feed industry.


Keywords


Adhesion ability; antibiotic susceptibility; E. faecalis; tolerance.

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References


Abriouel, H., Omar, N.B., Molinos, A.C., Lopez, R.L., Grande, M.J.,

et al. (2008). Comparative analysis of genetic diversity and incidence

of virulence factors and antibiotic resistance among enterococcal

population from raw fruit and vegetable foods, water and soil, and clinical

samples. International Journal of Food Microbiology, 123:38-49.

Ayeni, F., Sanchez, B., Adeniyi, B.A., de los Reyes-Gavilan, C.G.,

Margolles, A. et al. (2011). Evaluation of the functional potential of

Weissella and Lactobacillus isolates obtained from Nigerian traditional

fermented foods and cows intestine. International Journal of Food

Microbiology, 147:97-104.

Bao, Y., Zhang, Y., Zhang, Y., Liu, Y., Wang, S., et al. (2010).

Screening of potential probiotic properties of Lactobacillus fermentum

isolated from traditional dairy products. Food Control, 21:695-701.

Barrow, P.A., Brooker, B.E., Fuller, R. & Newport, M.J. (1980).

The attachment of bacteria to the gastric epithelium of the pig and its

importance in the microecology of the intestine. Journal of Applied

Microbiology, 48:147-154.

Bhardwaj, A., Gupta, H., Kapila, S., Kaur, G., Vij, G. et al.

(2010). Safety assessment and evaluation of probiotic potential of

bacteriocinogenic Enterococcus faecium KH 24 strain under in vitro

and in vivo conditions. International Journal of Food Microbiology,

:156-164.

Bhatia, S.J., Kochar, N. & Abraham, P. (1989). Lactobacillus

acidophilus inhibits growth of Campylobacter pylori in vitro. Journal

of Clinical Microbiology, 27:2328-2330.

Cebrian, R., Banos, A., Valdivia, E., Perez-Pulido, R., Martinez-

Bueno, M. et al. (2012). Characterization of functional, safety, and

probiotic properties of Enterococcu faecalis UGRA10, a new AS-48-

producer strain. Food Microbiology, 30:59-67.

Giraffa, G. (2003). Functionality of enterococci in dairy products.

International Journal of Food Microbiology, 88:215-222.

Gomes, B.C., Esteves, C.T., Palazzo, I.C.V., Darini, A.L.C., Felis,

G.E., et al. (2008). Prevalence and characterization of Enterococcus

spp. isolated from Brazilian foods. Food Microbiology, 25:668-675.

Guerra, N.P., Bernardez, P.F., Mendez, J., Cachaldora, P. & Castro,

L.P. (2007). Production of four potentially probiotic lactic acid bacteria

and their evaluation as feed additives for weaned piglets. Animal Feed

Science and Technology, 134:89-107.

Guo, X.H., Kim, J.M., Nam, H.M., Park, S.Y. & Kim, J.M. (2010).

Screening lactic acid bacteria from swine origins for multistrain

probiotics based on in vitro functional properties. Anaerobe, 16:321-

doi:10.1016/2010/03006

Guo, Z., Wang, J., Yan, L., Chen, W., Liu, X. & Zhang, H. (2009). In

vitro comparison of probiotic properties of Lactobacillus casei Zhang, a

potential new probiotic, with selected probiotic strains. LWT-Food

Science and Technology, 42:1640-1646. doi:10.1016/2009/05025

Gupta, H. & Malik, R.K. (2007). Incidence of virulence in

bacteriocin-producing enterococcal isolates. Le Lait, 87:587-601

doi:10.1051/2007/031

Hussain, R. & Ashfaq, M. (2009). Susceptibility of malthion-resistant

and susceptible Tribolium castaneum adults to abamectin, spinosad

and indoxacarb. Kuwait Journal of Science & Engineering, 36:113-

IDS number in Web of Science: 479YE, WOS in Web of Science:

Jurkovic, D., Krizkova, L., Dusinsky, R., Belicova, A., Sojka, M.,

et al. (2006). Identification and characterization of enterococci from

bryndza cheese. Letters in Applied Microbiology, 42:553-559.

Kailaspathy, K. & Rybka, S. (1997). Lactobacillus acidophilus and

Bifidobacterium spp.-their therapeutic potential and survival in yogurt.

Australian Journal of Dairy Technology, 52:8-35.

Kirtzalidou, E., Pramateftaki, P., Kotsou, M. & Kyriacou, A.

(2011). Screening for lactobacilli with probiotic properties in the infant

gut microbiota. Anaerobe, 17:440-44. doi:10.1016/2011/05007

Klingberg, T.D., Axelsson, L., Naterstad, K., Elsser, D. & Budde,

B.B. (2005). Identification of potential probiotic starter cultures for

Scandinavian-type fermented sausages. International Journal of Food

Microbiology, 105:419-431. doi:10.1016/2005/03020

Laukova, A., Strompfova, V. & Ouwehand, A.C. (2004). Adhesion

properties of enterococci to intestinal mucus of different hosts.

Veterinary Research Communications, 28:647-655.

Lin, W.H., Yu, B., Jang, S.H. & Tsen, H.Y. (2007). Different probiotic

properties for Lactobacillus fermentum strains isolated from swine and

poultry. Anaerobe, 13:107-113. doi: 10.1016/2007/04006

Linaje, R., Coloma, M.D., Perez-Martinez, G. & Zuniga, M. (2004).

Characterization of faecal enterococci from rabbits for the selection of

probiotics strains. Journal of Applied Microbiology, 96:761-771. doi:

1111/2004/02191

Marcinakova, M., Klingberg, T.D., Laukova, A. & Budde, B.B.

(2010). The effect of pH, bile and calcium on the adhesion ability of

probiotic enterococci of animal origin to the porcine jejuna epithelial

cell line IPEC-J2. Anaerobe, 16:120-124. doi:10.1016/2009/05001

Matsumoto, M., Ohishi, H. & Benno, Y. (2004). H+-ATPlase activity

in bifidobacterium with special reference to acid tolerance. International

Journal of Food Microbiology, 93:109-113. doi:10.1016/2003/10009

Nueno-Palop, C. & Narbad, A. (2011). Probiotic assessment of

Enterococcus faecalis CP 58 isolated from human gut. International

Journal of Food Microbiology, 145:390-394.

Pan, X., Chen, F., Wu, T., Tang, H. & Zhao, Z. (2009). The acid, bile

tolerance and antimicrobial property of Lactobacillus acidophilus NIT.

Food Control, 20:598-602.

Piano, M.D., Morellic, L., Strozzib, G.P., Allesina, S., Barbab, M., et

al. (2006). Probiotics: from research to consumer. Digestive and Liver

Disease, 38:S248-S255.

Rebucci, R., Sangalli, L., Fava, M., Bersani, C., Cantoni, C. &

Baldi, A. (2007). Evaluation of functional aspects in Lactobacillus

strains isolated from dry fermented sausages. Journal of Food Quality,

:187-201.

Rinkinen, M., Westermack, E., Salminen, S. & Ouwehand, A.C.

(2003). Absence of host specificity for in vitro adhesion of probiotic

lactic acid bacteria to intestinal mucus. Veterinary Microbiology, 97:55-

Ripamonti, B., Agazzi, A., Bersani, C., Dea, P.D., Pecorini,

C., et al. (2011). Screening of species-specific lactic acid bacteria

for veal calves multi-strain probiotic adjuncts. Anaerobe, 17:97-105.

doi:10.1016/2011/05001

Rui-Moyano, S., Martin, A., Benito, M.J., Perez-Nevado, F. &

Cordoba, M.G. (2008). Screening of lactic acid bacteria and

bifidobacteria for potential probiotic use in Iberian dry fermented

sausages. Meat Science, 80:715-721. doi:10.1016/2008/03011

Silvia, A. & Nakaia, J.K.S. (2003). Validation of bacterial growth inhibition models based on molecular properties of organic acids.

International Journal of Food Microbiology, 86:249-255.

Sogaard, M., Norgaard, M. & Schonheyder, H. (2007). First

notification of positive blood cultures: high accuracy of the Gram strain

report. Journal of Clinical Microbiology, 45:1113-1117.

Strompfova, V., Laukova, A. & Ouwehand, A.C. (2004). Selection

of enterococci for potential canine probiotic additives. Veterinary

Microbiology, 100:107-114.

Tan, Q., Xu, H., Aguilar, Z.P., Peng, S., Dong, S., et al. (2013). Safety

assessment and probiotic evaluation of Enterococcus Faecium YF5

isolated from sourdough. Journal of Food Science, 78:M587-M593.

Tsai, C.C., Lin, P.P. & Hsieh, Y.M. (2008). Three Lactobacillus strains

from healthy infant stool inhibit enterotoxigenic Escherichia coli grown

in vitro. Anaerobe, 14:61-67. doi:10.1016/2007/11003

Ventrua, M., Canchaya, C., van Sinderen, D., Fitzgerald, G.F. &

Zink, R. (2004). Bifidobacterium lactis DSM 10140: identification

of the atp (atpBEFHAGDC) operon and analysis of its genetic

structure, characteristics, and phylogeny. Applied and Environmental

Microbiology, 70:3110-3121. doi:10.1128/2004/705


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