Evaluation of partial characteristics of the strain Enterococcus faecalis P3 isolated from peacock feces in vitro
Keywords:
Adhesion ability, antibiotic susceptibility, E. faecalis, tolerance.Abstract
In this study, the potential probiotic properties such as NaCl tolerance, acid tolerance, simulated gastro-intestinal juicetolerance, adhesion ability to Hep-2 cells, antibiotic susceptibility and antimicrobial activity against selected pathogens, of the strain E. faecalis P3 identified in our group were evaluated in vitro. The results showed that E. faecalis P3 had
tolerance to NaCl. The viability was kept higher than 8 log CFU/mL at 2-5 % concentration of NaCl during 24 h incubation. E. faecalis P3 grew well in acid condition (pH 1.8-6.2) for 24 h incubation. The viable numbers decreased with the increase of incubation time in simulated gastro-intestinal juices. The viable numbers were kept higher than 10 log CFU/mL in simulated gastric juice (SGJ) pH 2.5 after 2 h incubation. Furthermore, E. faecalis P3 was able to adhere
to Hep-2 cells. The results of antibiotic susceptibility indicated E. faecalis P3 was sensitive to most of the clinically important antibiotics. E. faecalis P3 had good inhibition ability on Staphylococcus aureus. In conclusion, E. faecalis P3 appeared to be a good candidate for use as a probiotic agent in food or feed industry.
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