Estimation of the phenolics content of St. John’s wort (Hyperıcum perforatum L.) grown under different water and salt levels based on reflectance spectroscopy
Keywords:
Phenolics, Modeling, Hypericum, Water stress, SaltAbstract
Abstract
Accumulation of phenolics were examined in greenhouse-grown Hypericum perforatum plants as effected by three different salts which were MgSO4, CaCl2, and NaCl in the salt concentrations of 0.03 (control), 1, 2.5, 4, and 8 dS/m for each salt. Three different water stress levels, which were 80, 100, and 120% were applied to all three salts mentioned above. Multi regression analyses were performed to describe the effects of water stress and salt levels on phenolics accumulation. As a result of ANOVA and multi-regression analysis, it was found that there was close relationship between actual and predicted phenolic contents in Hypericum perforatum. HPLC analyses were used to determine quercetin, quercitrin, isoquercetin, hyperoside, rutin, and chologenic acid contents. The relationships between water stress and different salt levels, and phenolics accumulation were determined by spectral reflectance values. The r values for quercetin, quercitrin, isoquercetin, hyperoside, rutin, and chologenic acid were determined as 0.83, 0.82, 0.91, 0.88, 0.84, and 0.87, respectively. All r-values and standard errors of the equations were found to be significant at the p<0.001 level.
References
REFERENCES
Ayan, A.K. & Cirak, C. 2008. Hypericin and pseudohypericin contents in some Hypericum species growing in Turkey, Pharmaceutical Biology. 46 (4): 288-291.
Benkiki, N., Kabouche, Z., Tillequin, F., Verite, P., Chosson, E. & Seguin, E. 2003. A new polyisoprenylated phloroglucinol derivativefrom Hypericum perfoliatum (Clusiaceae). Z Naturforsch 58:655–658.
Bray, E.A., Bailey-Serres J. & Weretinlnyk, E. 2000. Responses to a biotic stresses, in:W.Gruissem, B.Buchannan,R.Jones (Eds),Biochemistry and molecular biology of plants. America Society of Plant Physiologists, Rockville, pp. 1158-1249.
Cirak, C., Odabas, M.S., & Ayan, A.K. 2005. Leaf area prediction model for summer Snowflake (Leucojum aestivum L.) // International Journal of Botany. Vol. 1, iss. 1, p. 12–14.
Cirak, C., Bertoli, A., Pistelli, L. & Seyis, F. 2010. Essential oil composition and variability of Hypericum perforatum from wild populations of northern Turkey. Pharmaceutical Biology. 48(8): 906-914.
Cirak, C., Radusiene, J., Çamaş, N., Çalışkan, Ö. & Odabaş, M.S. 2013. Changes in the contents of main secondary metabolites in two Turkish Hypericum species during plant development. Pharmaceutical Biology. 51(3): 391-399.
Cirak, C., Radusiene, J., Janulis, V. & Ivanauskas, L. 2007. Chemical constituents of some Hypericum species growing in Turkey. Journal of Plant Biology. 50(6) : 632-635.
Cirak, C., Radusiene, J., Sağlam, B. & Janulis, V. 2007. Variation of bioactive substances and morphological traits in Hypericum perforatum populations from Northern Turkey. Biochemical Systematics and Ecology. 35: 403-409.
Cirak, C., Radusiene, J., Stanius, Z., Çamaş, N., Çalışkan, Ö. & Odabaş, M.S. 2012. Secondary metabolites of Hypericum orientale L. growing in Turkey: variation among populations and plant parts. Acta Physiologia Plantarum. 34:1313–1320.
Cirak, C., Sağlam, B., Ayan, A.K. & Kevseroğlu K. 2006. Morphogenetic and diurnal variation of hypericin in some Hypericum species from Turkey during the course of ontogenesis. Biochemical Systematics and Ecology. 34 : 1-13.
Copeman, R.H., Martin, C.A. & Stutz, J.C. 1996. Tomato growth in response to salinity and mycorrhizal fungi from saline or non-saline soils. Hortscience 31(3): 341–344.
Erper, I., Turkkan, M. & Odabas, M.S. 2011. The mathematical approach to the effect of potassium bicarbonate on mycelial growth of Sclerotinia sclerotiorum and Rhizoctonia solani in vitro. Zemdirbyste=Agriculture. 98(2):195-204.
Flowers, T.J. & Flowers, S.A. 2005. Why does salinity pose such a difficult problem for plant breeders? Agricultural Water Management, 78:15-24.
Kara, B. & Kara, N. 2010. Effect of different salinity (NaCl) concentrations on the first development stages of root and shoot organs of wheat. Anadolu J. Agric. Sci., 25(1):pp. 37-43.
Lauchli, A. & Epstein, E. 1990. Plant responses to saline and sodic conditions. In K.K. Tanji (ed). Agricultural salinity assessment and management. ASCE manuals and reports on engineering practice No, 71. pp 113–137 ASCE New York.
Marschner, H. & Romheld, V. 1994. Strategies of plants for acquisition of iron. Plant Soil, 165: 375-388.
Morales, C., Cusido, R.M., Palazon, J. & Bonfill, M. 1993. Tolerance of mint plants to soil salinity. J. Indian Soc. Soil Sci., 44(1), pp.184-186.
Odabas, M.S., Radusiene, J. & Camas, N. 2009. The quantitative effects of temperature and light intensity on hyperforin and hypericins accumulation in Hypericum perforatum L. // Journal of Medicinal Plants Research.Vol. 3, iss. 7, p 519–525
Odabas, M.S., Radugiene, J., Camas, N., Janulis, V., Ivanauskas, L. & Cirak, C. 2009. The quantitative effects of temperature and light intensity on hyperforin and hypericins accumulation in Hypericum perforatum L. Journal of Medicinal Plants Research. (3)7: 519-525.
Odabas, M.S., Radusiene, J., Cirak, C. & Camas, N. 2008. Prediction Modelsfor the Phenolic Contents in Some Hypericum Species from Turkey. Asian J. Chem. 20(6): 4792-4802.
Page, A.L., Chang, A.C. & Adriano, D.C. 1990. Agricultural salinity assessment stresses and management deficiencies and toxicities of trace elements, in: tanj, K.K. (Eds), Manuals and Reports on Eng. Practice No.71, New York, pp. 138-160.
Prusinkiewicz, P. 2004. Modeling plant growth and development // Current Opinion in Plant Biology. Vol. 7, p. 79–83.
Rhoades, J.D. 1974. Drainage for salinity control. In: Drainage for agriculture. (Ed.): J. Van Schilfgarde. ASA Monograph no. 17, Amer. Soc. Agronomy, Madison, Wis., 433-467.
Said-Al Ahl, H.A.H, Meawad, A.A., Abou-Zeid, E.N. & Ali, M.S. 2010. Response of different basil varieties to soil salinity. Int. Agrophysics, 24, pp.183-188.
Shao, H.B, Chu, L.Y. & Jaleel, C.A. 2008. Water-deficiet stress-induced anatomical changes in higher plants, C.R.Biologies, 331(3):215-225.
Tanji, K.K. 1990. Nature and extent of agricultural salinity. Agricultural Salinity Assessment and Management. ASCE Manuals and Reports on Engineering Practice No.71, American Society of Civil Eng., New York, 1-17.
Wu, G., Zhang, C., Chu, L.Y. & Shao, H.B. 2007. Responses of higher plants to a biotic stress an agricultural sustainable development. J. Plant Interactions, 2(3): 135-147.
Yildirim, E. & Taylor, A.G. 2005. Effect of biological treatments on growth of bean plants under salt stress. Annual Report of the Bean Improvement Cooperative, March 2005, 48: 176-177.
