An efficient in vitro propagation system for Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant
Keywords:Plant regeneration, shoot tip explants, carbon sources, CNP ratio, acclimatization
An efficient micropropagation system is needed for successful conservation and improvement of plants by biotechnological means. Nanorrhinum ramosissimum (Wall.) Betsche, a traditionally acclaimed medicinal plant has been selected for the present study. Shoot tip explants exhibited the best organogenetic response when inoculated on ½ strength Murashige and Skoog (MS) medium supplemented with Kinetin (KN) and Indole-3-acetic acid (IAA) (0.5 mg/Leach). Shoot organogenesis was further enhanced when the multiplication medium was fortified with dextrose (1%) (2.6 shoots/explant; mean length 7.9 cm). The influence of Carbon:Nitrogen:Phosphorous (CNP) proportion on in vitro propagation demonstrated that the control combination i.e. 1:1:1 ratio as utilized in standard ½ strength MS medium was the most appropriate combination with induction of 2.38 shoots/explant having a mean length of 6.88 cm. All the regenerated shoots formed complete plantlets; however, the best rooting frequency was achieved on ½ strength MS medium containing IAA (0.5 mg/L). Four week old in vitro rooted plantlets were acclimatized with 95% of survival under greenhouse conditions. The regeneration protocol developed in this study provides a basis for germplasm conservation of this elite medicinal plant.
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