An efficient in vitro propagation system for Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant




Plant regeneration, shoot tip explants, carbon sources, CNP ratio, acclimatization


An efficient micropropagation system is needed for successful conservation and improvement of plants by biotechnological means. Nanorrhinum ramosissimum (Wall.) Betsche, a traditionally acclaimed medicinal plant has been selected for the present study. Shoot tip explants exhibited the best organogenetic response when inoculated on ½ strength Murashige and Skoog (MS) medium supplemented with Kinetin (KN) and Indole-3-acetic acid (IAA) (0.5 mg/Leach). Shoot organogenesis was further enhanced when the multiplication medium was fortified with dextrose (1%) (2.6 shoots/explant; mean length 7.9 cm). The influence of Carbon:Nitrogen:Phosphorous (CNP) proportion on in vitro propagation demonstrated that the control combination i.e. 1:1:1 ratio as utilized in standard ½ strength MS medium was the most appropriate combination with induction of 2.38 shoots/explant having a mean length of 6.88 cm. All the regenerated shoots formed complete plantlets; however, the best rooting frequency was achieved on ½ strength MS medium containing IAA (0.5 mg/L). Four week old in vitro rooted plantlets were acclimatized with 95% of survival under greenhouse conditions. The regeneration protocol developed in this study provides a basis for germplasm conservation of this elite medicinal plant. 

Author Biography






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