Biodegradation of Tannins from Polluted Sources Using Natural Enzyme

Authors

  • Sanaa Elsayed Tork King Abdulaziz university

Keywords:

Cryptococcus, Tannin acyl hydrolase, Tannic acid, SDS-PAGE, 18S rRNA

Abstract

This study aimed to characterize an efficient tannin- degrading microorganism. Ten isolates were screened with medium supplemented with tannic acid. The highest tannase producer was the yeast; therefore, it was selected for more detail studies. Based on its morphological and biochemical characteristics, as well as phylogenetic analysis, this isolate was identified as Cryptococcus sp. NRC10 with homology level (99.9%).  The optimization of factors affecting tannase production was studied. It was shown that medium II at 45°C for 24 h at pH 5 was the most favourable conditions required for maximum enzyme production. Glucose and NH4Cl proved to be the best carbon and nitrogen source, respectively. The purified enzyme was characterized. It was found that the best conditions were 0.20% of tannic acid and 0.15 ml tannase concentration at a temperature of 45°C after 10 minutes with pH 6. The addition of Mn2+ stimulates the activity of the enzyme. The purified enzyme was used for the degradation of Tannins in Red Sea water and in green tea leaves samples. It was noted a decrease in the amount of tannin after the addition of tannase enzyme. In conclusion, the enzyme has a role in the removal of Tannins in water and soil.

References

Aguilar, C. N., Rodríguez, R., Gutiérrez-Sánchez, G., Augur, C., Favela-Torres, E., Prado-Barragan, L. A., Ramírez-Coronel, A., and Contreras-Esquivel, J. C. (2007). Microbial tannases: advances and perspectives. Applied microbiology and biotechnology 76, 47-59.

Aithal, M., and Belur, P. D. (2013). Enhancement of propyl gallate yield in nonaqueous medium using novel cell-associated tannase of Bacillus massiliensis. Preparative Biochemistry and Biotechnology 43, 445-455.

Akroum, S. (2014). Aspergillus niger Growth on polyphenolic carbon sources and optimization of the tannase production. G. J. Bio-Science and Biotechnol. 3, 246-249.

Aly, M. M., Tork, S., Al-Garni, S. M., and Kabli, S. A. (2011). Chitinolytic enzyme production and genetic improvement of a new isolate belonging to Streptomyces anulatus. Annals of Microbiology 61, 453-461.

Anitha, A., and Arunkumar, D. (2013). Extraction, partial purification and characterization of tannase enzyme from Mucor sp. Nehru E-Journal 1, 25-29.

Aoki, K., Shinke, R., and Nishira, H. (1976). Purification and some properties of yeast tannase. Agricultural and Biological Chemistry 40, 79-85.

Banerjee, A., Jana, A., Pati, B. R., Mondal, K. C., and Mohapatra, P. K. D. (2012). Characterization of tannase protein sequences of bacteria and fungi: an in silico study. The protein journal 31, 306-327.

Barthomeuf, C., Regerat, F., and Pourrat, H. (1994). Production, purification and characterization of a Tannase from Aspergillus niger LCF 8. Journal of Fermentation and Bioengineering 77, 320-323.

Beena, P. ( 2010). Production, purification, genetic characterization and application studies of tannase enzyme from marine fungus Aspergillus awamori. Ph.D. thesis,. Cochin University of Science and Technology. Cochin, Kerala, India.2010.

Beniwal, V., Chhokar, V., Singh, N., and Sharma, J. (2010). Optimization of process parameters for the production of tannase and gallic acid by Enterobacter cloacae MTCC 9125. J Am Sci 6, 389-397.

Bhardwaj, R., Singh, B., and Bhat, T. K. (2003). Purification and characterization of tannin acyl hydrolase from Aspergillus niger MTCC 2425. J Basic Microbiol 43, 449-61.

Bialek, R., Fischer, J., Feucht, A., Najvar, L. K., Dietz, K., Knobloch, J., and Graybill, J. R. (2001). Diagnosis and monitoring of murine histoplasmosis by a nested PCR assay. J Clin Microbiol 39, 1506-9.

Bradoo, S., Gupta, R., and Saxena, R. K. (1997). Parametric optimization and biochemical regulation of extracellular tannase from Aspergillus japonicus. Process Biochemistry 32, 135-139.

Chowdhury, S. P., Khanna, S., Verma, S. C., and Tripathi, A. K. (2004). Molecular diversity of tannic acid degrading bacteria isolated from tannery soil. J Appl Microbiol 97, 1210-9.

Chung, K. T., Wong, T. Y., Wei, C. I., Huang, Y. W., and Lin, Y. (1998). Tannins and human health: a review. Crit Rev Food Sci Nutr 38, 421-64.

Dave, A., Modi, H., and Chavada, N. (2011). Study And Isolation Of Tannase Enzyme Production Bacteria From Tea Waste Dump Soil Site. IRJPAS 2, 0976-6936.

Deschamps, A., Lebeault, J., and Otuk, G. (1983). Production of tannase and degradation of chestnut [Castanea crenata] tannin by bacteria. Journal of Fermentation Technology (Japan).

El-Fouly, M., El-Awamry, Z., Shahin, AA., , El-Bialy, H., Naeem, E., and El-Saeed, G. (2010). Biosynthesis and characterization of Aspergillus niger AUMC 4301 tannase. J. Am. Sci. 6., 709-721.

El-Tanash, A., Sherief, A., and Nour, A. (2012). Optimization the hydrolysis process of tannic acid for gallic acid production by tannase of Aspergillus awamori using response surface methodology. Innovative Romanian Food Biotechnology 10, 9-17.

Frutos, P., Hervás, G., Giráldez, F. J., and Mantecón, A. R. (2004). Review. Tannins and ruminant nutrition. 2004 2, 12.

Goel, G., Puniya, A. K., Aguilar, C. N., and Singh, K. (2005). Interaction of gut microflora with tannins in feeds. Naturwissenschaften 92, 497-503.

Gupta, R., Bradoo, S., and Saxena, R. (1997). Rapid purification of extracellular tannase using polyethylene glycol–tannic acid complex. Letters in applied microbiology 24, 253-255.

Iqbal, H., Kapoor, A (2012). Tannin Degradation Efficiency of Tannase Produced By Trichoderma harzianum MTCC 10841 and its Biochemical Properties. Int. J. Life Sci. Biotechnol. Pharma Res. 1, 2250 - 3137.

Iwamoto, K., Tsuruta, H., Nishitaini, Y., and Osawa, R. (2008). Identification and cloning of a gene encoding tannase (tannin acylhydrolase) from Lactobacillus plantarum ATCC 14917(T). Syst Appl Microbiol 31, 269-77.

Jana, A., Maity, C., Halder, S. K., Pati, B. R., Mondal, K. C., and Mohapatra, P. K. D. (2012). Rapid screening of tannase producing microbes by using natural tannin. Brazilian journal of microbiology 43, 1080-1083.

Kar, B., Banerjee, R., and Bhattacharyya, B. C. (2003). Effect of additives on the behavioural properties of tannin acyl hydrolase. Process Biochemistry 38, 1285-1293.

Khanbabaee, K., and van Ree, T. (2001). Tannins: classification and definition. Nat Prod Rep 18, 641-9.

Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-5.

Lal, D., and Gardner, J. (2012). Production, characterization and purification of tannase from Aspergillus niger. European Journal of Experimental Biology 2, 1430-1438.

Madeira, J., Valdo, J., Macedo, J. A., and Macedo, G. A. (2011). Detoxification of castor bean residues and the simultaneous production of tannase and phytase by solid-state fermentation using Paecilomyces variotii. Bioresource technology 102, 7343-7348.

Mata-Gomez, M., Rodriguez, L. V., Ramos, E. L., Renovato, J., Cruz-Hernandez, M. A., Rodriguez, R., Contreras, J., and Aguilar, C. N. (2009). A novel tannase from the xerophilic fungus Aspergillus niger GH1. J Microbiol Biotechnol 19, 987-96.

Mohapatra, P. D., Maity, C., Rao, R., Pati, B., and Mondal, K. (2009). Tannase production by Bacillus licheniformis KBR6: Optimization of submerged culture conditions by Taguchi DOE methodology. Food research international 42, 430-435.

Mondal, K. C., Banerjee, D., Banerjee, R., and Pati, B. R. (2001). Production and characterization of tannase from Bacillus cereus KBR9. J Gen Appl Microbiol 47, 263-267.

Nadaf, N., and Ghosh, J. (2011). Production, purification and characterization of tannase from Rhodococcus NCIM, 2891. Current Research Journal of Biological Sciences 3, 246-253.

Negi A, S. N., Pant R, Singh MF. (2012). Determination of total phenolic content of the stem bark of Bauhinia variegata Linn.; an approach to standardization. Pharma Research 7, 16-22.

Parales, R. E., and Haddock, J. D. (2004). Biocatalytic degradation of pollutants. Curr Opin Biotechnol 15, 374-9.

Patel, R., Dodia, M., and Singh, S. P. (2005). Extracellular alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.: Production and optimization. Process Biochemistry 40, 3569-3575.

Pepi, M., Lampariello, L. R., Altieri, R., Esposito, A., Perra, G., Renzi, M., Lobianco, A., Feola, A., Gasperini, S., and Focardi, S. E. (2010). Tannic acid degradation by bacterial strains Serratia spp. and Pantoea sp. isolated from olive mill waste mixtures. International Biodeterioration & Biodegradation 64, 73-80.

Ramirez-Coronel, M. A., Viniegra-Gonzalez, G., Darvill, A., and Augur, C. (2003). A novel tannase from Aspergillus niger with beta-glucosidase activity. Microbiology 149, 2941-6.

Rangari, V. D. (2007). Pharmacognosy Phytochemistry. 1, Career Publication, Nashik,19.

Reddy, M., and Kumar, C. ( 2011 ). Production of tannase by isolated Aspergillus terrus under solid state fermentation. International Journal of Pharmacological Research Development 3, 41-49.

Rodriguez, H., de las Rivas, B., Gomez-Cordoves, C., and Munoz, R. (2008). Characterization of tannase activity in cell-free extracts of Lactobacillus plantarum CECT 748T. Int J Food Microbiol 121, 92-8.

Sabu, A., Kiran, G., and Pandey, A. (2005). Purification and characterization of tannin acyl hydrolase from Aspergillus niger ATCC 16620. . Food Technology and Biotechnology 43.

Sambrook, J., Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press , Cold Spring Harbor, NY, USA, 3rd edition.

Saxana, R., Sharmila, P., and Singh, V. (1995). Microbial degradation of tannins. Progress in Industrial Microbiology. Biotransformations: Microbial Degradation of Health Risk Compounds. . Elsevier Science B.V 32, 259-270.

Saxena, R. K., Sharmila, P., and Singh, V. P. (1995). Microbial degradation of tannins. In "Progress in Industrial Microbiology" (V. P. Singh, ed.), Vol. 32, pp. 259-270. Elsevier.

Singh, V. P. (2002). Biotransformations of tannery wastes. In "Progress in Industrial Microbiology" (S. Ved Pal and S. Raymond D, eds.), Vol. 36, pp. 279-290. Elsevier.

Sivashanmugam, K., and Jayaraman, G. (2011). Production and partial purification of extracellular tannase by Klebsiella pneumoniae MTCC 7162 isolated from tannery effluent. . African Journal of Biotechnology. 10, 1364-1374.

Ueda, S., Nomoto, R., Yoshida, K., and Osawa, R. (2014). Comparison of three tannases cloned from closely related lactobacillus species: L. Plantarum, L. Paraplantarum, and L. Pentosus. BMC Microbiol 14, 87.

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Published

2020-10-03