Design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosis


  • sina vakili
  • gholamerza asadikaram kerman university of medical sciences
  • Masoud Torkzadeh-Mahani
  • Alimohammad Behroozikhah
  • Mohammad Hadi Nematollahi
  • Amir Savardashtaki



nanobiosensor, brucellosis, LSPR, gold nanoparticles


In this study, a localized surface plasmon resonance (LSPR) nanobiosensor was designed to quantify anti-Brucella antibody in the human sera. Smooth Lipopolysaccharide (LPS) was extracted from Brucella melitensis via modified hot phenol water method and fixed on the surface of the gold nanoparticles by covalent interactions. In order to obtain the best performance from the designed probe, the ratio of LPS to gold nanoparticle was optimized. Dynamic light scattering was used for characterization of the probe. The reduction of the LSPR peak at 600 nm was used to quantify the amount of captured anti-Brucella antibody. Finally, satisfactory results were obtained when the nanobiosensor was used to analyze the control and patient sera for the presence of anti-Brucella antibodies.


ALMUNEEF, M., MEMISH, Z., BALKHY, H., ALOTAIBI, B., ALGODA, S., ABBAS, M. & ALSUBAIE, S. (2004). Importance of screening household members of acute brucellosis cases in endemic areas. Epidemiology and infection, 132, 533-540.

ARAJ, G. F. (2010). Update on laboratory diagnosis of human brucellosis. International journal of antimicrobial agents, 36, S12-S17.

BELLAN, L. M., WU, D. & LANGER, R. S. (2011). Current trends in nanobiosensor technology. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 3, 229-246.

COBLEY, C. M., CHEN, J., CHO, E. C., WANG, L. V. & XIA, Y. 2011. Gold nanostructures: a class of multifunctional materials for biomedical applications. Chemical Society Reviews, 40, 44-56.

FARIDLI, Z., MAHANI, M., TORKZADEH-MAHANI, M. & FASIHI, J. (2016). Development of a localized surface plasmon resonance-based gold nanobiosensor for the determination of prolactin hormone in human serum. Analytical biochemistry, 495, 32-36.

FRANCO, M. P., MULDER, M., GILMAN, R. H. & SMITS, H. L. (2007). Human brucellosis. The Lancet infectious diseases, 7, 775-786.

GÓMEZ, M. C., NIETO, J. A., ROSA, C., GEIJO, P., ESCRIBANO, M. A., MUNOZ, A. & LÓPEZ, C. (2008). Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic. Clinical and Vaccine Immunology, 15, 1031-1033.

HOLZINGER, M., LE GOFF, A. & COSNIER, S. (2014). Nanomaterials for biosensing applications: a review. Frontiers in chemistry, 2, 63.

HONG, Y., HUH, Y.-M., YOON, D. S. & YANG, J. (2012). Nanobiosensors based on localized surface plasmon resonance for biomarker detection. Journal of Nanomaterials, 2012, 111.

KATTAR, M. M., ZALLOUA, P. A., ARAJ, G. F., SAMAHA-KFOURY, J., SHBAKLO, H., KANJ, S. S., KHALIFE, S. & DEEB, M. (2007). Development and evaluation of real-time polymerase chain reaction assays on whole blood and paraffin-embedded tissues for rapid diagnosis of human brucellosis. Diagnostic microbiology and infectious disease, 59, 23-32.

MARTIN, M. N., BASHAM, J. I., CHANDO, P. & EAH, S.-K. (2010). Charged gold nanoparticles in non-polar solvents: 10-min synthesis and 2D self-assembly. Langmuir, 26, 7410-7417.

MITKA, S., ANETAKIS, C., SOULIOU, E., DIZA, E. & KANSOUZIDOU, A. (2007). Evaluation of different PCR assays for early detection of acute and relapsing brucellosis in humans in comparison with conventional methods. Journal of clinical microbiology, 45, 1211-1218.

MORENO, E., PITT, M., JONES, L., SCHURIG, G. & BERMAN, D. (1979). Purification and characterization of smooth and rough lipopolysaccharides from Brucella abortus. Journal of bacteriology, 138, 361-369.

NIELSEN, K. & YU, W. (2010). Serological diagnosis of brucellosis. Prilozi, 31, 65-89.

PADILLA POESTER, F., NIELSEN, K., ERNESTO SAMARTINO, L. & LING YU, W. 2010. Diagnosis of brucellosis. The Open Veterinary Science Journal, 4.

SEPÚLVEDA, B., ANGELOMÉ, P. C., LECHUGA, L. M. & LIZ-MARZÁN, L. M. (2009). LSPR-based nanobiosensors. Nano Today, 4, 244-251.