Variation in antioxidant potential of Vigna unguiculata grown in pure and amended soil
Keywords:Sewage sludge, Agriculture, Antioxidant contents
AbstractThe variation in antioxidant capacity on Vigna unguiculata was determined by carrying out the pot experiment in pure and in amended soil. After amending the soil, the suitability of sewage sludge for agricultural purposes was assessed. V. unguiculata was grown in six pots with different soil amendments including control soil, 20%, 40%, 60% and 80% sludge proportion as well as pure sludge. Antioxidant contents increased in linear fashion in all plant parts when grown in soil amendments possessing 20%, 40% and 60% sludge proportion; With 80% sludge proportion as well as in pure sludge, antioxidant contents decreased due to enhanced oxidative stress caused by higher concentrations of metal ions present with in the sewage sludge. Antioxidant potential assessed by DPPH assay, ABTS±scavenging activity and lipid peroxidation method showed significant improvement up to the soil amendments with 60% sludge proportion and decreased in higher amendments.
Ahldberg, G., Gustafrron, O. & Wedel, P. (2006). Leaching of metals from sewage sludge during one year and their relationship to particle size. Environmental Pollution, 144: 545-553.
Antolin, M.C., Pascual, I., Garcia, C., Polo, A. & Sanchez-Diaz, M. (2005). Growth, yield and solute content of barley in soils treated with sewage sludge under semiarid Mediterranean conditions. Field Crops Research, 94: 224-237.
Ayrton, A.D., Lewis, D.F.V., Walker, R. & Ioannides, C. (1992). Antimutagenicity of ellagic acid towards the food mutagen IQ: Investigation into possible mechanisms of action. Food and Chemical Toxicology, 30: 289-295.
Bharagava, R.N., Chandra, R. & Rai, V. (2008). Phytoextraction of trace elements and physiological changes in Indian mustard plants (Brassica nigra L.) grown in post methanated distillery effluent (PMDE) irrigated soil. Bioresource Technology, 99: 8316-8324.
Bukhari, S.M., Feuerherm, A.J., Boulfrad, F., Zlatković, B., Johansen, B. & Simić, N. (2014). Anti-inflammatory and antioxidant activities of Sclerochloa dura (Poaceae). Journal of the Serbian Chemical Society, 79: 779–791.
Fridovich, I. (1978). The biology of oxygen radicals. Science, 201: 875-880.
Iqbal, S., Bhanger, M.I. & Anwar, F. (2005). Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chemistry, 93: 265–272.
Iqbal, S., Bhanger, M.I. & Anwar, F. (2007). Antioxidant properties and components of bran extracts from selected wheat varieties commercially available in Pakistan. LWT-Food Science and Technology, 40: 361–367.
Marti, M.C., Camejo, D., Neves, Fernandez-Garcia, Rellan-Alveraz, R., Marques, S., Sevillia, F. & Jimenez, A. (2009). Effect of oil refinery sludges on the growth and antioxidant system of alfalfa plants. Journal of Hazardous Materials, 171: 879-885.
Nice, D.J. & Robinson, D.S. (1992). Inhibition of lipid autoxidation by bovine superoxide dismutase. Food Chemistry, 45: 99-103.
Pietta, G.P. (2000). Flavonoids as antioxidants. Journal of Natural Products, 63: 1035- 1042.
Polshettiwar, S.A., Ganjiwale, R.O., Wadher, S.J. & Yeole, P.G. (2007). Spectrophotometric estimation of total tannins in some ayurvedic eye drops. Indian Journal of Pharmaceutical Sciences, 69: 574-576.
Singh, R.P. & Agarawal, M. (2008). Potential benefits and risks of land application of sewage sludge. Waste Management, 28: 347-358.
Sinha, S., Gupta, A.K. & Bhatt, K. (2007). Uptake and translocation of metals in fenugreek grown on soil amended with tannery sludge: Involvement of antioxidants. Ecotoxicology and Environmental Safety, 67: 267-277.
Sinha, S., Singh, S., Mallick, S. & Sinam, G. (2009). Role of antioxidants in Cr tolerance of three crop plants: Metal accumulation in seeds. Ecotoxicology and Environmental Safety, 72: 1111-1121.
Tewari, A., Singh, R., Singh, N.K. & Rai, U.N. (2008). Amelioration of municipal sludge by Pistia stratiotes L.: Role of antioxidant enzymes in detoxification of metals. Bioresource Technology, 99: 8715-8721.