Lipid lowering effect of Eurycoma longifolia Jack aqueous root extract in hepatocytes.


  • Pei J. Lim Department of Exercise Science, Sports Centre, University of Malaya
  • Chye S. Gan Department of Molecular Medicine,Faculty of Medicine,University of Malaya
  • Ashril Yusof Department of Exercise Science,Sports Centre,University of Malaya


Eurycoma longifolia Jack, lipid, metabolic disease, testosterone, Tongkat Ali


An aqueous extract of Eurycoma longifolia Jack, or natively known in Malay as Tongkat Ali, roots is traditionally used to overcome plethora of health conditions especially andrological maladies. As compiling evidences corroborate the therapeutic effects of this plant extract as efficacious concoction, it is noteworthy that novel potential for the use of the plant extract in regulating fat metabolism is being unravelled. Due to studies that investigated the effects of Tongkat Ali on in vivo models and clinical subjects where the measurable parameters were the end stage of response, this study adopts an in vitro model to investigate whether Tongkat Ali root extract possesses lipid lowering potential or the changes was a side-effect of the supplementation. Human hepatic cell line WRL-68 was used in this study and treated with various concentrations (1, 2, 4 and 8 µg/ml) of the aqueous Tongkat Ali root extract were conducted on fatty liver cells. Results demonstrated reduced intracellular fatty acids build up, up to ~72% reduction, in treated cells when compared to those untreated control cells. This study therefore, unravelled the novel potential of intracellular lipid lowering effect of Tongkat Ali. The finding therefore, warrants further studies to elucidate the mechanisms that explain the phenomenon.


Ang, H. H. & Sim, M. K. (1997) Eurycoma longifolia Jack enhances libido in sexually experienced male rats. Experimental Animals, 46: 287-90.

Ang, H. H. & Sim, M. K. (1998a) Eurycoma longifolia increases sexual motivation in sexually naive male rats. Archives of Pharmacal Research, 21: 779-81.

Ang, H. H. & Sim, M. K. (1998b) Eurycoma longifolia JACK and orientation activities in sexually experienced male rats. Biological and Pharmaceutical Bulletin, 21: 153-5.

Bhasin, S., Woodhouse, L., Casaburi, R., Singh, A. B., Bhasin, D. et al. (2001) Testosterone dose-response relationships in healthy young men. American Journal of Physiology- Endocrinology and Metabolism, 281: E1172-81.

Chan, K. L., Low, B. S., Teh, C. H. & Das, P. K. (2009) The effect of Eurycoma longifolia on sperm quality of male rats. Natural Product Communications, 4: 1331-6.

Feldstein, A. E., Werneburg, N. W., Canbay, A., Guicciardi, M. E., Bronk, S. F. et al. (2004) Free fatty acids promote hepatic lipotoxicity by stimulating TNF-alpha expression via a lysosomal pathway. Hepatology, 40: 185-94.

Gan, C. S., Yusof, R. & Othman, S. (2015) Different serotypes of dengue viruses differently regulate the expression of the host cell antigen processing machinery. Acta Tropica, 149: 8-14.

Hamzah, S. & Yusof, A. (2003) The ergogenic effects of Eurycoma longifolia Jack: a pilot study. British Journal of Sports Medicine, 37: 464-470.

Kotirum, S., Ismail, S. B. & Chaiyakunapruk, N. (2015) Efficacy of Tongkat Ali (Eurycoma longifolia) on erectile function improvement: systematic review and meta-analysis of randomized controlled trials. Complementary Therapies in Medicine, 23: 693-8.

Kuo, P. C., Shi, L. S., Damu, A. G., Su, C. R., Huang, C. H. et al. (2003) Cytotoxic and antimalarial beta-carboline alkaloids from the roots of Eurycoma longifolia. Journal of Natural Products, 66: 1324-7.

Nurhanan, M. Y., Azimahtol Hawariah, L. P., Mohd Ilham, A. & Mohd Shukri, M. A. (2005) Cytotoxic effects of the root extracts of Eurycoma longifolia Jack. Phytotherapy Research, 19: 994-6.

Park, S., Nhiem, N. X., Kiem, P. V., Minh, C. V., Tai, B. H. et al. (2014) Five new quassinoids and cytotoxic constituents from the roots of Eurycoma longifolia. Bioorganic & Medicinal Chemistry Letters, 24: 3835-40.

Solomon, M. C., Erasmus, N. & Henkel, R. R. (2014) In vivo effects of Eurycoma longifolia Jack (Tongkat Ali) extract on reproductive functions in the rat. Andrologia, 46: 339-48.

Woodhouse, L. J., Gupta, N., Bhasin, M., Singh, A. B., Ross, R. et al. (2004) Dose-dependent effects of testosterone on regional adipose tissue distribution in healthy young men. Journal of Clinical Endocrinology & Metabolism, 89: 718-26.

Zakaria, Y., Rahmat, A., Pihie, A. H., Abdullah, N. R. & Houghton, P. J. (2009) Eurycomanone induce apoptosis in HepG2 cells via up-regulation of p53. Cancer Cell International, 9: 16.