Polyhydroxyalkanotes (PHAs) production by using canola oil as carbon source from bacteria isolated near paper pulp industry
Abstract
Samples were taken from paper pulp and mixed organic wastes of an industry for isolation of PHA producing bacterial strains. Quantitative analysis for PHA for bacterial strains was carried out by direct addition of sodium dodecyl sulphate (SDS) method. High PHA production ability was found in six strains belonging to Pseudomonas, Klebsiella, Bacillus, Streptococcus, Staphylococcus and Escherichia genera. The PHA production optimization of these six stains was done at various (NH4)2SO4 concentrations (0.2 %, 0.4 %, and 0.6%), pH (5, 6, and 7) and temperatures (4oC, 37oC, 45oC). Strain WC20 belonging to Pseudomonas sp. was found to be a potential PHA producer at 37 oC, at 0.2 % of (NH4)2SO4, using glucose as carbon source (PHA % ~28.35) and Canola Oil (PHA % ~16.06). PCR amplification of the phaC gene was also performed.
References
Akiyama, M., Tsuge, T. & Doi, Y. 2003. Environmental life cycle comparison of polyhydroxyalkanoates
produced from renewable carbon resources by bacterial fermentation. Polymer Degradation and
Stability 80:183-194.
Ali, I. & Jamil, N. 2014. Enhanced biosynthesis of poly (3-hydroxybutyrate) from potato starch by
Bacillus cereus strain 64-INS in a laboratory scale fermenter. Preparative Biochemistry and
Biotechnology 44: 822-833.
An derson, A. J. & Dawes, E. A.1990. Occurrence, metabolism, metabolic role, and industrial uses of
bacterial polyhydroxyalkanoates. Microbiological reviews 54:450-472.
Au subel, F. M. 2002. Short protocols in molecular biology. Wiley, New York
Cappuccino, J.G. & Sherman, N.2007. Microbiology: A Laboratory Manual. 7th ed. Pearson Education.
Casini, E., de Rijk, T. C. & de Ward, P.1997. Synthesis of poly(hydroxyalkanoate) from hydrolyzed
linseed oil. Journal of Polymer and Environment 5:153–158.
Ch audhry, W. N., Jamil, N., Ali, I., Ayaz, M. H. & Hasnain, S.2011. Screening for polyhydroxyalkanoate
(PHA)-producing bacterial strains and comparison of PHA production from various inexpensive
carbon sources. Annals of Microbiology 61:623-629.
De marco, S. M.2006. Advances in Polyhydroxyalkanoate Production in Bacteria for Biodegradable
Plastic. MMG 445 BasicBiotechnology eJournal 1:1-4.
Di n, M., Fadil, M., Ujang, Z., Van Loosdrecht, M. & Ahmad, M. A.2012. Polyhydroalkanoates (PHAs)
Production from Saponified Sunflower Oil in Mixed Cultures under Aerobic Condition. Jurnal
Teknologi 48:1–19.
Eg gink, G., Waard, P. D. & Huijberts, G. N.1995. Formation of novel poly (hydroxyalkanoates) from
long-chain fatty acids. Canadian Journal of Microbiology 41:14-21.
Es kin, N. M. & McDonald, B.1991. Canola oil. Nutrition Bulletin 16:138-146.
Fu kui, T. & Doi, Y.1998. Efficient production of polyhydroxyalkanoates from plant oils by Alcaligenes
eutrophus and its recombinant strain. Applied Microbiology and Biotechnology 49:333-336.
Ho cking, P. & Marchessault, R.1994. Biopolyesters. In: Griffin GJL (ed) Chemistry and technology of
biodegradable polymers. London, UK: Blackie Academic & Professional., pp 48-96.
Ja u, M. H., Yew, S. P., Toh, P. S., Chong, A. S., Chu, W. L., Phang, S. M., Najimudin, N. & Sudesh, K.
Biosynthesis and mobilization of poly(3-hydroxybutyrate) [P(3HB)] by Spirulina platensis.
International Journal of Biological Macromolecules 36:144-151.
Ka har, P., Tsuge, T., Taguchi, K. & Doi, Y.2004. High yield production of polyhydroxyalkanoates from
soybean oil by Ralstonia eutropha and its recombinant strain. Polymer Degradation and Stability
:79-86.
Ki m, M., Cho, K-S., Ryu, H. W., Lee, E. G. & Chang, Y. K.2003. Recovery of poly (3-hydroxybutyrate)
from high cell density culture of Ralstonia eutropha by direct addition of sodium dodecyl sulfate.
Biotechnology Letters 25:55-59.
Ko bayashi, G., Shiotani, T., Shima, Y. & Doi, Y.1994. Biosynthesis and Characterization of Poly
(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Oils and Fats by Aeromonas sp. OL-338 and
Aeromonas sp. FA-440. In: Doi Y and Fukuda K. (eds) Biodegradable Plastics and Polymers.
Elsevier, Amsterdam, pp 410-416.
Le e, S. & Choi, J.1999. Polyhydroxyalkanoates: biodegradable polymer. In: Demain A L, Davies J E and
Atlas R M. (eds) Manual of Industrial Microbiology and Biotechnology, vol 2. 2 edn., Washington,
D.C. American Society of Microbiology, pp 616-627.
Lee, S. Y., Wong, H. H. & Choi, J. I.2000. Production of medium-chain-length polyhydroxyalkanoates
by high cell density cultivation of Pseudomonas putida under phosphorus limitation. Biotechnology
and Bioengineering 68:466–470.
Le e, S. H., Oh, D. H., Ahn, W. S., Lee, Y., Choi, Ji. & Lee, S. Y.2000. Production of poly
(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high-cell-density cultivation of Aeromonas
hydrophila. Biotechnology and Bioengineering 67:240-244.
Le e, S. Y.1996. Bacterial polyhydroxyalkanoates. Biotechnology and Bioengineering 49:1-14.
Lo o, C-Y., Lee, W-H., Tsuge, T., Doi, Y. & Sudesh, K.2005. Biosynthesis and characterization of poly
(3-hydroxybutyrate-co-3-hydroxyhexanoate) from palm oil products in a Wautersia eutropha
mutant. Biotechnology Letters 27:1405-1410.
Lo o, C-Y. & Sudesh, K.2007. Polyhydroxyalkanoates: bio-based microbial plastics and their properties.
Malaysian Polymer Journal 2:31-57.
López-Cuellar, M. R., Alba-Flores, J., Rodríguez, J. N. & Pérez-Guevara, F.2011. Production of
polyhydroxyalkanoates (PHAs) with canola oil as carbon source. International Journal of Biological
Macromolecules 48: 74-80.
Ma del Rocio, L-C., Noel, G-R. J. & Fermín, P-G.2007. Production of polyhydroxyalcanoates by
Wautersia eutropha using vegetable oils as carbon source. Journal of Biotechnology 131:S156.
Mi fune, J., Nakamura, S. & Fukui, T.2008. Targeted engineering of Cupriavidus necator chromosome
for biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Canadian
Journal of Chemistry 86:621-627.
Pu rushothaman, M., Anderson, R., Narayana, S. & Jayaraman, V.2001. Industrial byproducts as
cheaper medium components influencing the production of polyhydroxyalkanoates (PHA)–
biodegradable plastics. Bioprocess and Biosystems Engineering 24:131-136.
Rathinasabapathy, A., Ramsay, B. A., Ramsay, J. A. & Pérez-Guevara, F.2013. A feeding strategy for
incorporation of canola derived medium-chain-length monomers into the PHA produced by wildtype
Cupriavidusnecator. World Journal of Microbiology and Biotechnology 1-8.
Sa mbrook, J. & Russell, D. W.2001. Molecular cloning: a laboratory manual. Volume 1–3. Cold Spring
Harbor, New York: Cold Spring Harbor Laboratory Press,
Shang, L., Jiang, M., Yun, Z., Yan, H. Q. & Chang, H. N.2008. Mass production of medium-chainlength
poly (3-hydroxyalkanoates) from hydrolyzed corn oil by fed-batch culture of Pseudomonas
putida. World Journal of Microbiology and Biotechnology 24: 2783-2787.
Sp iekermann, P., Rehm, B., Kalscheuer, R. & Baumeister, D.1999. A sensitive, viable-colony staining
method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids
and other lipid storage compounds. Archives for Microbiology 171:73-80.
Tan, I. K. P., Sudesh, K. & Theanmalar, M.1997 .Saponified palm kernel oil and its major free fatty
acids as carbon substrates for the production of polyhydroxyalkanoates in Pseudomonas putida
PGA1. Applied Microbiology and Biotechnology 47:207–211.
Ts uge, T.2002. Metabolic improvements and use of inexpensive carbon sources in microbial production
of polyhydroxyalkanoates. Journal of Bioscience and Bioengineering 94:579-584.
