Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive



  • Shahzad Mahmood GC University lahore
  • Memuna G. Shahid Department of Botany, Government College University, Katchery Road, Lahore-54000, Pakistan
  • Muhammad Nadeem Food and Biotechnology Research Centre (FBRC), Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Ferozepur Road, Lahore-54600, Pakistan
  • Muhammad S. A. Ahmad Department of Botany, University of Agriculture, University Road Faisalabad-38000, Pakistan
  • Muhammad Irfan Departmentof Biotechnology, University of Sargodha, Sargodha-40100, Pakistan



The present study deals with the production of phytase from Aspergillus niger using solid-state fermentation (SSF). Appropriate concentration of different components of the growth medium played an important role for the higher production of phytase. Maximum enzyme activity (265 IU/g) was obtained, when the fungus was grown in the fermentation medium containing, (% w/w) glucose 1, NH4NO3 0.5, MgSO4.7H2O, KCl  and FeSO4.7H2O 0.1 each, after 5 days of incubation time using pH 6 at 35 °C incubation temperature. Phytase produced during present study was also supplemented as poultry feed additive and given to broiler chickens for 5 weeks. The results exhibited that there was increase in body weight gain (BWG) of chicks for experimental group T3 (3240 g) in comparison to control group (2090 g). The current study indicated that phytase can effectively be supplemented in the broilers diet for better growth performance, recommending the use of phytase as useful additive in poultry feed formulation.