Enhanced phytase production by Aspergillus niger mutants in solid state fermentation

DOI: 10.48129/kjs.15995

Authors

  • Shahzad Mahmood Dept. of Botany, Government College University, Katchery Road, Lahore-54000, Pakistan
  • Memuna G. Shahid Dept. of Botany, Government College University, Katchery Road, Lahore-54000, Pakistan
  • Muhammad Nadeem Food and Biotechnology Research Centre (FBRC), Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Ferozepur Road, Lahore-54600, Pakistan
  • Rubina Nelofer Food and Biotechnology Research Centre (FBRC), Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Ferozepur Road, Lahore-54600, Pakistan
  • Muhammad Irfan Dept. of Biotechnology, University of Sargodha, Sargodha-40100, Pakistan

DOI:

https://doi.org/10.48129/kjs.15995

Abstract

The present research work was conducted to improve the phytase production by genetic alteration of Aspergillus niger with induced mutagenesis using solid state fermentation. Strain improvement was carried out in the presence of ultra violet (UV) irradiation and ethyl-methane sulphonate (EMS) (0.5% v/v) treatments for various time intervals. We reported an improved strain of Aspergillus niger designated as UV-3 mutant producing a zone of hydrolysis of about 40 mm, in comparison to parent strain (26 mm). The highest enzyme activity was found to be 547.64 IU/g for UV-3 mutant compared to parent strain which showed 406.45 IU/g of enzyme activity. Thus, physical and chemical mutagenesis was proved as an effective technique for the improvement of strain and ultimately for enhanced and economical phytase production for different industrial applications.

Author Biographies

Shahzad Mahmood, Dept. of Botany, Government College University, Katchery Road, Lahore-54000, Pakistan

 

 

Memuna G. Shahid, Dept. of Botany, Government College University, Katchery Road, Lahore-54000, Pakistan

 

 

Muhammad Nadeem, Food and Biotechnology Research Centre (FBRC), Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Ferozepur Road, Lahore-54600, Pakistan

 

 

Rubina Nelofer, Food and Biotechnology Research Centre (FBRC), Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Ferozepur Road, Lahore-54600, Pakistan

 

 

Muhammad Irfan, Dept. of Biotechnology, University of Sargodha, Sargodha-40100, Pakistan

 

 

Published

08-01-2023