Involvement of miR-3648 mediated APC2 dysregulation in early onset and breast cancer progression

DOI: 10.48129/kjs.15891

Abstract

Multiple cancers arise due to aberrations in the Wnt signaling pathway. Several miRNAs modulate the integral components of the Wnt signaling pathway. miR-3648 is a human-specific miRNAs that is of particular interest due to its minimum off-targeting. In this study, we investigate the expression of miR-3648 and APC2 in breast cancer patients of Pakistan. Possible association of miR-3648 and APC2 expression with clinico-pathological features and breast cancer subtypes was observed in tissue samples by means of quantitative real time PCR. Our results showed that miR-3648 is relatively downregulated in Luminal A subtype, with corresponding upregulation of APC2 in these patients. Moreover, the transcript levels of both miR-3648 and APC2 are inversely regulated in breast cancer affected women who presented with early disease onset, pre-menopause, low tumor grade, early clinical stage, absence of nodal invasion and metastasis, therefore, affirming molecular interplay of these molecules in breast cancer development and progression.