Involvement of miR-3648 mediated APC2 dysregulation in early onset and breast cancer progression
Multiple cancers arise due to aberrations in the Wnt signaling pathway. Several miRNAs modulate the integral components of the Wnt signaling pathway. miR-3648 is a human-specific miRNAs that is of particular interest due to its minimum off-targeting. In this study, we investigate the expression of miR-3648 and APC2 in breast cancer patients of Pakistan. Possible association of miR-3648 and APC2 expression with clinico-pathological features and breast cancer subtypes was observed in tissue samples by means of quantitative real time PCR. Our results showed that miR-3648 is relatively downregulated in Luminal A subtype, with corresponding upregulation of APC2 in these patients. Moreover, the transcript levels of both miR-3648 and APC2 are inversely regulated in breast cancer affected women who presented with early disease onset, pre-menopause, low tumor grade, early clinical stage, absence of nodal invasion and metastasis, therefore, affirming molecular interplay of these molecules in breast cancer development and progression.