Insilico analysis on the complex relationship among antibiotic resistance, virulence genes and insertion sequences in Pseudomonas aeruginosa

10.48129/kjs.14591

Authors

  • Santhiya Kalimuthu Karpagam Academy of Higher Education

DOI:

https://doi.org/10.48129/kjs.14591

Abstract

Multidrug-resistant (MDR) Pseudomonas aeruginosa is the most common nosocomial pathogens which cause several infections in humans, posing a serious health threats worldwide. The present study aims at the insilico analysis to understand the correlation among antibiotic resistance genes (ARG) and virulence factors (VF) in association with Insertion Sequences (IS) in P.aeruginosa.  In this study, 56 complete plasmids of P.aeruginosa obtained from the NCBI database. The CARD tool is used to identify the ARG genes among the P.aeruginosa sequences. Then, VG analyzer and ISsaga tools were used to detect the virulence genes and Insertion sequences respectively in the sorted plasmids. Among 56 plasmids, 22 had prefect antibiotic resistant genes. Sulphonamide and beta lactam resistance was being the major ARG among the plasmids. The virulence gene fil, pil and xcp secretion system was found to be prominent. The Insertion sequences like ISKpn6, ISKpn7, ISPa16, ISPa17, IS6100, ISPt3, ISPen2, TnAs3, IS222 were found in the plasmids were mostly belong to IS21 and IS6 family. The ARG and IS patterns was seen in 7 plasmids whereas such pattern of occurrence was less when we compare with VR. The correlation analysis among ARG, VR and IS showed that ISs has major influence in P.aeruginosa genome taken for the study rather than virulence factors. Therefore, understanding of infectious bacterial profile in relation with pathogenicity islands and mobile elements is need for an hour to get an insight on its dissemination to control its spread all over the world.

Keywords: Antibiotic resistance, Insertion sequence, Mobile elements, Pseudomonas, Virulence genes

Multidrug-resistant (MDR) Pseudomonas aeruginosa is the most common nosocomial pathogens which cause several infections in humans, posing a serious health threats worldwide. The present study aims at the insilico analysis to understand the correlation among antibiotic resistance genes (ARG) and virulence factors (VF) in association with Insertion Sequences (IS) in P.aeruginosa.  In this study, 56 complete plasmids of P.aeruginosa obtained from the NCBI database. The CARD tool is used to identify the ARG genes among the P.aeruginosa sequences. Then, VG analyzer and ISsaga tools were used to detect the virulence genes and Insertion sequences respectively in the sorted plasmids. Among 56 plasmids, 22 had prefect antibiotic resistant genes. Sulphonamide and beta lactam resistance was being the major ARG among the plasmids. The virulence gene fil, pil and xcp secretion system was found to be prominent. The Insertion sequences like ISKpn6, ISKpn7, ISPa16, ISPa17, IS6100, ISPt3, ISPen2, TnAs3, IS222 were found in the plasmids were mostly belong to IS21 and IS6 family. The ARG and IS patterns was seen in 7 plasmids whereas such pattern of occurrence was less when we compare with VR. The correlation analysis among ARG, VR and IS showed that ISs has major influence in P.aeruginosa genome taken for the study rather than virulence factors. Therefore, understanding of infectious bacterial profile in relation with pathogenicity islands and mobile elements is need for an hour to get an insight on its dissemination to control its spread all over the world.

Keywords: Antibiotic resistance, Insertion sequence, Mobile elements, Pseudomonas, Virulence genes

Published

06-07-2022

Issue

Section

Biology