Carbon nanodots-based C-dips for rapid colorimetric detection of clinically important metal ions

DOI: 10.48129/kjs.13997

Authors

  • Sumaira Nishat Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan
  • Malik Waseem Abbas Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan
  • Fazli Rabbi Awan Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan
  • Razium Ali Soomro Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan

DOI:

https://doi.org/10.48129/kjs.13997

Abstract

Owing to unique physical and optical properties, fluorescent carbon dots (C-dots) are used in various biomedical applications. In this study, C-dots have been integrated as sensing agent on paper surface for colorimetric determination of clinically important analytes. Polyacrylamide-based C-dots coated C-dips showed visible color change from white to brown for iron (Fe2+, Fe3+) sample. Moreover, iron and copper (Cu2+) samples showed significant quenching of C-dots under UV. Images captured with/without UV were analyzed to study the relationship between different iron and copper concentrations and colorimetric intensity values. Color intensity and fluorescence showed a linear relationship with concentrations of iron and copper (Range: 1×10-4 to 1×10-2 M, Linearity: R2 = 0.94). LOD from the paper sensor showed a sensitivity of 3.89×10-5 μM, 4.65×10-5 μM and 6.7×10-5 μM for Fe3+, Fe2+ and Cu2+ respectively, reflecting its reliability as low-cost sensing solution to analyze clinically relevant analytes as reported in current study.

Author Biographies

Sumaira Nishat, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan

 

 

Malik Waseem Abbas, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan

 

 

Fazli Rabbi Awan, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan

 

 

Razium Ali Soomro , Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan

 

 

Published

01-06-2023