Antimicrobial resistance pattern and phenotypic detection of ESBL- and MBL- producing Pseudomonas aeruginosa isolated from indoor-patients suffering ear discharge

DOI: 10.48129/kjs.10773

Authors

  • Farooq Ali Department of Microbiology, Hazara University Mansehra.
  • Qismat Shakeela Department of Microbiology, Hazara University Mansehra, 21300, KP, Pakistan
  • Bibi Uzma Department of Pathology, Ayub Medical College Abbottabad, 22010, KP, Pakistan
  • Asma Bibi Institute of Zoonosis, Anhui Medical University, Anhui, 230032, P. R China
  • Bushra Najeeb Department of Pathology, Ayub Medical College Abbottabad, 22010, KP, Pakistan
  • Atta ur Rahman Department of Microbiology, Hazara University Mansehra, 21300, KP, Pakistan
  • Mubassir Shah Department of Microbiology, Hazara University Mansehra, 21300, KP, Pakistan
  • Shehzad Ahmed Department of Microbiology, Hazara University Mansehra, 21300, KP, Pakistan

DOI:

https://doi.org/10.48129/kjs.10773

Abstract

A multidrug-resistant bacterium due to its intrinsic resistance nature and beta-lactamases production, Pseudomonas aeruginosa (P. aeruginosa) can colonize ubiquitously and is thus associated with life-threating bacterial infections. The study was aimed to inspect phenotypic detection and antimicrobial resistance pattern of ESBL- and MBL-producing P. aeruginosa strains. 220 clinical specimens were collected from indoor patients with ear discharge were inoculated on suitable culture media. Antimicrobial resistance pattern of all isolates was investigated employing Kerby-Bauer disc diffusion method. Double-disc synergy test and Imipenem-EDTA test were used respectively to detect ESBL- and MBL-producing strains. From 220, 37 (16.82%) clinical specimens confirmed the growth of P. aeruginosa. In comparison to female (43.24%), male population (56.76%) was more prevalent. Out of 37 positive cases, 16 (43.24%) strains were detected as ESBL-producers, while 7 (18.92%) as MBL producers. Cefotaxime (100%) was found the most resisted antibiotic by isolates, followed by aztreonam (91.89%), gentamycin (86.49%), ceftriaxone (83.78%) and tazobactam/piperacillin (64.87%), while the lowest resistance was observed against imipenem (21.63%) and meropenem (51.36%). Furthermore, ESBL-producing strains revealed high resistance against cefotaxime (100%), ceftriaxone (93.75%), and carbenicillin (87.5%), while MBL-producing strains were completely resistance to imipenem, meropenem, cefotaxime and carbenicillin, followed by gentamicin (85.71%) and amikacin (75.42%). Our study concluded that strains of P. aeruginosa producing ESBL and MBL enzymes were mostly resistant to the drugs of choice, which puzzle the physicians to treat infections caused by P. aeruginosa. So, it is needed to study the resistant pattern of P. aeruginosa in order to recommend proper medication.

Published

26-12-2021

Issue

Section

Biology