Genetic variations in TAOK1, TMTC4, NTF3, and CNTN1 and their association with noise-induced hearing loss



Noise-induced hearing loss (NIHL) affects more than 10% of the adult population in developed countries, especially those with high noise levels. In this study, the objective is to examine the effect of the variability in sensitivity to noise exposure of genetic variations in the TAOK1, TMTC4, NTF3 and CNTN1 genes. A total of 323 people in 7 different businesses were included in the scope of the research. For a total of eight SNP regions, including SNP regions from the TAOK1, TMTC4, NTF3 and CNTN1 genes; SNP genotyping was performed using the ABI 7500 Real-Time PCR System with 96 wells according to rs191010498 TAOK1 C/T, rs1469121309 TAOK1 A/T, rs763560019 TMTC4 T/A, rs1484543954 TMTC4 G/C, rs1334049414 NTF3 T/C, rs1438314793 NTF3 G/A, rs762307549 CNTN1 G/A,T, rs745850349 CNTN1 T/A, Custom TaqMan® SNP Genotyping Assay and standard protocol. It was observed that the CT heterozygous frequencies of the TAOK1 gene, age, working time, working in a noisy environment over 85 db(A) (p<0.05) were found to be significantly higher in the hearing loss group compared to the control group. In Turkish population, sensitivity to noise-induced hearing loss was observed in five SNP regions out of eight SNP regions of TAOK1, TMTC4, NTF3 and CNTN1 genes, while TAOK1, TMTC4 and CNTN1 genes were also associated with hearing loss, however, no statistically significant difference was found in the NTF3 genes found among the rare variants. The results showed that the TAOK1-rs191010498 (p=0.01), TAOK1-rs1469121309 (p=0.03), TMTC4-rs763560019 (p=0.01), CNTN1-rs762307549 (p=0.01), CNTN1- rs745850349 (p=0.03) polymorphisms were associated with NIHL. Identification of TAOK1, TMTC4, CNTN1 polymorphisms is important in terms of providing a new contribution to the genetic roles associated with hearing loss.