In vitro controlled drug delivery of cationic substituted hydroxyapatite nanoparticles; enhanced anti-chelating and antibacterial response

Authors

  • Muhammad S Asghar Dept. of Physics, University of Wah, Wah Cantt, Pujab, Pakistan
  • Uzma Ghazanfar Dept. of Physics, University of Wah, Wah Cantt, Pujab, Pakistan
  • Muhammad Idrees Dept. of Biosciences, University of Wah, Wah Cantt, Pujab, Pakistan
  • Muhammad S. Irshad Ministry-of-Education Key Laboratory for the Green Preparation and Application ofFunctional Materials, School of Materials Science and Engineering, Hubei University, Wuhan, P. R. China
  • Zeenat Haq Dept. of Biosciences, University of Wah, Wah Cantt, Pujab, Pakistan
  • Muhammad Q. Javed FBRC, Pakistan Council of Scientific and Industrial Research, Lahore, Pujab, Pakistan
  • Syed Z. Hassan Dept. of Electronics Engin., University of Engineering and Technology, Taxila, Punjab, Pakistan.
  • Muhammad Rizwan Dept. of Physics, University of Wah, Wah Cantt, Pujab, Pakistan

DOI:

https://doi.org/10.48129/kjs.18957

Abstract

Hydroxyapatite (HAp) is naturally found in the bones and is used to deliver antimicrobials and antibacterial agents such as chlorhexidine and gentamicin to the bone. The unique ability of hydroxyapatite crystalline lattice regarding the substitution of calcium ions with an excessive cationic species is being widely explored for utilization in nanoscale biomedicine. This study covers, the in vitro controlled drug delivery of cationic substituted hydroxyapatite (Nix=0.3,0.5 HAp) nanoparticles loaded with widely consumed drug, ciprofloxacin. Our results indicate that the cationic substitution of nickel in Hydroxyapatite increased the percentage of ciprofloxacin loading and release in a controlled mode of action. Cationic substituted hydroxyapatite (Nix=0.3,0.5 HAp) nanoparticles loaded with ciprofloxacin have shown antioxidant activity, prolonged release of ciprofloxacin and strong antibacterial activity against Escherichia coli (ATCC 35218) and Pseudomonas aeruginosa (ATTC 27853) strains. The Antibacterial properties of the Nickel and ciprofloxacin doped Hydroxyapatite along with sustained and prolonged release of Hydroxyapatite associated ciprofloxacin would be helpful for effective management and prevention of osteomyelitis and dental infections due to Escherichia coli and Pseudomonas aeruginosa.

Author Biographies

Muhammad S Asghar, Dept. of Physics, University of Wah, Wah Cantt, Pujab, Pakistan

Department of Physics, University of Wah, Wah Cantt, 47040, Pakistan

Muhammad Idrees, Dept. of Biosciences, University of Wah, Wah Cantt, Pujab, Pakistan

Department of Biosciences, University of Wah, Wah Cantt, 47040, Pakistan.

Muhammad S. Irshad, Ministry-of-Education Key Laboratory for the Green Preparation and Application ofFunctional Materials, School of Materials Science and Engineering, Hubei University, Wuhan, P. R. China

Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials Hubei University

Zeenat Haq, Dept. of Biosciences, University of Wah, Wah Cantt, Pujab, Pakistan

Department of Biosciences, University of Wah, Wah Cantt, 47040, Pakistan.

Muhammad Q. Javed, FBRC, Pakistan Council of Scientific and Industrial Research, Lahore, Pujab, Pakistan

Food and Biotechnology Research Center (FBRC), Pakistan Council of Scientific and Industrial Research, Lahore-54600, Pakistan.

Syed Z. Hassan, Dept. of Electronics Engin., University of Engineering and Technology, Taxila, Punjab, Pakistan.

Department of Electronics Engineering, University of Engineering and Technology, Taxila, 47080, Pakistan

Muhammad Rizwan, Dept. of Physics, University of Wah, Wah Cantt, Pujab, Pakistan

Department of Physics, University of Wah, Wah Cantt, 47040, Pakistan.

Published

17-10-2022

Issue

Section

Biology